|
KMID : 0380020050200020123
|
|
Korean Journal of Biotechnology and Bioengineering
2005 Volume.20 No. 2 p.123 ~ p.128
|
|
|
Integration of Immunohistochemical Reactions Into Electrochemical and Optical Analyses of Biochips
|
|
Choi Hyoung-Gil
Hong Eun-Kyoung
Lee Seung-Won
Yoon Hyun-Chul
|
|
|
Abstract
|
|
|
|
We have addressed two important issues of immunosensing biochips, including the construction of antibody functionalized suface for efficient affinity reactions and the development of a signal registration strategy that converts biospecific reactions into highly quantifiable electrochemical and/or optical signals. The developed immunoassay reaction is an integrated version of enzyme-mediated immunoprecipitaion reaction, which is widely used in immunohistochemistry, and electrochemical signaling reaction. For the evaluation of analytical performance of fabricated immunosensing biochips, signaling for mouse IgG in antiserum was conducted. Applications of the developed strategy have been found for the evaluation of histology chemicals and for the signal amplification for array-type biochip analysis.
|
|
|
KEYWORD
|
|
|
Biochip, biosensor, immunohistochemistry, immunoassay, electrochemical
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|